Biotransformation and oxidative stress responses in fish (Astyanax aeneus) inhabiting a Caribbean estuary contaminated with pesticide residues from agricultural runoff.

The estuarine ecosystem of Madre de Dios Lagoon (MDL), in the Caribbean Coast of Costa Rica, is exposed to contamination with pesticide residues coming from the upstream agricultural areas. Biomarkers can provide a better indication of the fitness of biota in real mixture exposure scenarios than traditional lethal dose toxicity measurements. Here, we measured biomarkers of biotransformation, oxidative stress, and neurotoxicity on Astyanax aeneus, an abundant fish species in MDL. Glutathione S-transferase activity (GST), catalase activity (CAT), lipid peroxidation (LPO), and cholinesterase activity (ChE) were measured in fish collected during seven sampling campaigns, carried out between 2016 and 2018. Pesticide residues were analyzed in surface water samples collected every time fish were sampled. Residues of 25 pesticides, including fungicides, insecticides, and herbicides, were detected. The biomarkers measured in A. aeneus varied along the sampling moments, with biotransformation and oxidative stress signals showing a coupled response throughout the assessment. Furthermore, significant correlations were established between three biomarkers (GST, LPO, and CAT) and individual pesticides, as well as between GST and LPO with groups of pesticides with shared biocide action. Among pesticides, insecticide residues had a major influence on the responses observed in fish. This work demonstrates the chronic exposure to pesticide residues in MDL and how such exposure is related to physiological responses in fish that can affect their health and potentially, the trophic networks. This early warning information should be considered to improve the protection of estuarine ecosystems in the tropics.

Ecological Integrity Impairment and Habitat Fragmentation for Neotropical Macroinvertebrate Communities in an Agricultural Stream.

The Volcán River watershed in the south Pacific of Costa Rica comprises forests, small urban settlements, cattle fields, and intensive agriculture (mostly pineapple and sugarcane). The ecological integrity and quality of its waters was assessed from 2011-2013 and 2018-2019 by means of physical-chemical parameters (pH, conductivity, temperature, DO, DBO, nitrate, total phosphorus, and pesticide residues) and benthic macroinvertebrate (MI) sampling in eight sites (Volcán, Cañas, and Ángel Rivers, and Peje and Maura streams), resulting in high ecological integrity in all sites except the Peje stream, which is polluted with nitrates and pesticides. Only in this stream was there a marked seasonal variation in the abundance of 16 MI families including Leptohyphidae, Leptophlebiidae, Philopotamidae, Glossossomatidae, and Corydalidae, among others, whose presence was limited exclusively to the dry season (December to April), disappearing from the stream in the rainy season, with corresponding peaks in nitrate (max 20.3 mg/L) and pesticides (mainly herbicides and organophosphate insecticides). The characteristics of the watershed, with large areas of forest and excellent water quality, allow for the re-colonization of organisms into the Peje stream; however, those organisms are incapable of development and growth, providing evidence of a contaminant-driven habitat fragmentation in this stream during the rainy season.

New species and records of Cloeodes Traver, 1938 (Ephemeroptera, Baetidae) from Costa Rica.

The nymph of Cloeodes danta sp. nov. is described from male and female nymphs collected from highland streams in the Caribbean Slope of the Costa Rica Central Volcanic Mountain Range. Adults are unknown. In addition, C. excogitatus and C. redactus are recorded for the first time in the country. Cloeodes danta sp. nov. can be differentiated from all described species by the predominantly brownish coloration on females and a similar coloration on males but with segments VII-IX light yellow and light brown, with no conspicuous marks or patterns; abundant scale-bases throughout most parts of the body; hindwings pads absent; the presence of three spines in the corners of the posterior margin of sternum III, and the posterior margin of tergum III with 28-30 spines on each side of the middle line (spine with a base width up to 0.5× spine length).

Insectos acuáticos y calidad del agua en la cuenca y embalse del río Peñas Blancas, Costa Rica

Aquatic insects and water quality in Peñas Blancas watershed and reservoir. The aquatic insects have been used to evaluate water quality of aquatic environments. The population of aquatic insects and the water quality of the area were characterized according to the natural and human alterations present in the study site. During the monthly-survey, pH, DO, temperature, water level, DBO, PO4 and NO3 were measured. Biological indexes (abundance, species richness and the BMWP-CR) were used to evaluate the water quality. No relation between environmental and aquatic insects was detected. Temporal and spatial differences attributed to the flow events (temporal) and the presence of Peñas Blancas reservoir (spatial). In the future, the investigations in Peñas Blancas watershed need to be focused on determining the real influence of the flows, sediment release and the possible water quality degradation because of agriculture activities. Rev. Biol. Trop. 59 (2): 635-654. Epub 2011 June 01.

La población de insectos acuáticos y la calidad del agua fueron caracterizadas tomando en cuenta las alteraciones humanas y naturales en el área de estudio. Durante los muestreos mensuales, el pH, OD, temperatura, nivel del agua, DBO, PO4 y NO3 fueron medidos. Los índices biológicos (abundancia, riqueza de especies y el BMWP-CR) se evaluaron para determinar la calidad del agua. No se detectaron relaciones entre las variables ambientales y los insectos acuáticos, pero las diferencias espaciales y temporales en la abundancia y riqueza fueron asociadas con las crecidas del río (temporal) y a la presencia del embalse Peñas Blancas (espacial). En el futuro las investigaciones en la cuenca Peñas Blancas deben de ser dirigidas a determinar la magnitud de la influencia de los caudales, liberación de sedimentos y la posible degradación de la calidad del agua por efluentes de aguas servidas que se lleguen a ubicar en la cuenca.